Fig. 2: Structural comparison of ATTR fibrils.
From: Structure of ATTRv-F64S fibrils isolated from skin tissue of a living patient
a Schematic representation of the secondary structure elements of ATTRv-F64S fibril. Top, ATTRv-F64S fibril β-sheet organization. Bottom, wild-type ATTR (PDB: 8ADE16) fibril shown for comparison. Arrows represent β-stands and dashed lines indicate unresolved regions of the fibril protein. Amino acid numbers of the transthyretin (TTR) protein are indicated below. The structure of ATTRv-F64S fibril is shown as a cartoon. b Hydrophobicity representation of a single fibril layer (top view). Hydrophobic grooves are shown with dashed lines. c Electrostatic surface representation of a single fibril layer (top view). Electrostatic potentials are contoured from -10 (red) to +10 kTe-1 (blue). Figures are prepared in Chimera X56. d Structural comparison of ATTR fibrils, including wild-type and variant forms. Left, Views of structural alignment between ATTRv-F64S fibril (tomato red) and ATTRwt fibrils (light blue) extracted from heart (PDB ID: 8ADE16, 8E7D18, 8G9R18, 8GBR18 and 8E7H18). Middle, Views of structural alignment between ATTRv-F64S fibril and cardiac ATTRv fibrils (wheat, PDB ID: 8PKE17, 8E7I, 6SDZ14, 8PKF17, 8E7E19, 8E7J19, 8TDN19, 8TDO19 and 8PKG17, including V20I, P24S, V30M, G47E, I84S and V122I mutants.). Right, structural alignment of ATTRv-F64S fibril with ATTRv-V30M fibril (sky blue, PDB ID: 7OB415) from the eye. The gate region is highlighted in pink.
