Fig. 2: CYP707A3-mediated water-soaking resistance depends on stomatal opening.
From: Humidity-driven ABA depletion determines plant-pathogen competition for leaf water
a, b Representative images of stomata (a) and stomatal aperture (b) in humidity-treated leaves. The indicated plants were exposed to moderate humidity (MH) or high humidity (HH) for 1 h. Bars represent the median (WT MH, n = 172; WT HH, n = 246; cyp707a1 cyp707a3 MH, n = 153; cyp707a1 cyp707a3 HH, n = 236; ost2-3D MH, n = 149; ost2-3D HH, n = 264; cyp707a1 cyp707a3 ost2-3D MH, n = 189; cyp707a1 cyp707a3 ost2-3D HH, n = 219). Scale bar = 10 μm. c Water-soaking in Pst ΔhrcC-inoculated leaves. The indicated plants were infiltrated with Pst ΔhrcC (OD600 = 0.02) and maintained under HH for 1 day. The number of water-soaked leaves was pooled from two independent experiments. Scale bar = 1 cm. d Bacterial growth in Pst ΔhrcC-inoculated leaves. The indicated plants were infiltrated with Pst ΔhrcC (OD600 = 0.02) and maintained under HH for 3 days. Bars represent means (n = 6). Sample size (n) in (b, d) indicate stomata and biological replicates, respectively. Different letters indicate statistically significant differences (P < 0.05; two-way ANOVA followed by Tukey’s test in (b); one-way ANOVA followed by Tukey’s test in (d)). WT wild-type, dpi days post-infiltration. Experiments in (b, d) were repeated twice with similar results.
