Fig. 4: CAMTA3 drives CYP707A3 expression to promote resistance to bacterial water-soaking under high humidity.
From: Humidity-driven ABA depletion determines plant-pathogen competition for leaf water
a Schematic diagram of the promoter regions of CYP707A3 and CYP707A1, highlighting the CGCG-box. b GUS expression driven by the CYP707A3 promoter, with or without CGCG boxes, in humidity-treated leaves. The indicated constructs were transiently expressed in NahG leaves via Agrobacterium infiltration. Infiltrated plants were maintained under moderate humidity (MH) for 24 h, then exposed to MH or high humidity (HH) for 0.5 h. Bars represent means (n = 3). c, d Expression levels of CYP707A3 and CYP707A1 in humidity-treated leaves of cngc (c) and camta3 (d) mutants. The indicated plants were exposed to MH or HH for 0.5 h. Bars represent means (n = 3). e ABA levels in humidity-treated leaves. The indicated plants were exposed to MH or HH for 2 h. Bars represent means (n = 5). f Stomatal aperture in humidity-treated leaves. The indicated plants were exposed to MH or HH for 1 h. Bars represent the median (WT MH, n = 224; WT HH, n = 247; Native #3 MH, n = 221; Native #3 HH, n = 222; A855V #23 MH, n = 226; A855V #23 HH, n = 223; A855V #27 MH, n = 220; A855V #27 HH, n = 235). g Water-soaking in Pst ΔhrcC-inoculated leaves. The indicated plants were infiltrated with Pst ΔhrcC (OD600 = 0.02) and maintained under HH for 1 day. The number of water-soaked leaves was pooled from two independent experiments. h, i Bacterial growth in Pst ΔhrcC-inoculated leaves. The indicated plants were infiltrated with Pst ΔhrcC (OD600 = 0.02) and maintained under HH (h) or MH (i) for 3 days. Bars represent means (n = 6). Sample size (n) in (b–e, h, i) and (f) indicate biological replicates and stomata, respectively. Different letters indicate statistically significant differences (P < 0.05; two-way ANOVA followed by Tukey’s test in (b–f); one-way ANOVA followed by Tukey’s test in (h, i)). WT wild-type, CAMTA3-A855V, a partial loss-of-function variant; dpi, days post-infiltration. Experiments in (b–d, f, h, i) were repeated twice with similar results.
