Fig. 5: Pst DC3000 blocks high humidity-driven plant transcriptome reprogramming via T3S effectors.
From: Humidity-driven ABA depletion determines plant-pathogen competition for leaf water
a Schematic overview of the experimental design for analyzing high humidity-responsive gene expression in Pst DC3000-inoculated leaves. Illustration of a syringe adapted from WDB Co., Ltd., Research Net (https://www.wdb.com/kenq/illust/syringe). b Bacterial titers in wild-type (WT) leaves infiltrated with Pst DC3000 or Pst ΔhrcC (OD600 = 0.02) at 8 h post-infiltration (hpi) under moderate humidity (MH). Bars represent means (n = 6). c Expression levels of CYP707A3, CYP707A1, and NCED3 in Pst DC3000-inoculated leaves following humidity treatment. WT plants were infiltrated with water (Mock) or the indicated Pst DC3000 strains (OD600 = 0.02), maintained under MH for 8 h, and then exposed to MH or high humidity (HH) for 0.5 h. Bars represent means (n = 3). d, e Transcriptome profiling of Pst DC3000-inoculated leaves following humidity treatment as described in (a, c). d Principal component analysis (PCA) of transcriptomic data. e K-means-clustering of the 2000 most variable genes. Sample size (n) indicates biological replicates. Different letters indicate statistically significant differences (P < 0.05; two-tailed Welch’s t-test in (b); two-way ANOVA followed by Tukey’s test in (c)). Experiments in (b, c) were repeated twice with similar results.
