Fig. 2: The macro-monocentromeres in Cha. luteum are satellite repeat-based.
A The monomer sequence of the Chama satellite repeat. The colored arrows indicate dyad symmetries. The 9-bp TTCGTACGA (underlined in black) is shared between the 60-bp Chama monomer and the 23-bp Chio monomer sequences31. Predicted hairpin loop structure formed by a Chama monomer. B Mitotic metaphase of Cha. luteum showing Chama (green) and telomere-specific (magenta) signals. C The genome proportion and normalized enrichment in CENH3-ChIPseq of the RepeatExplorer clusters. D Mapping of the CENH3-, H3K9me2-, and H3K4me2-ChIPseq reads, distribution of Chama satellite repeats, and of genic sequences to the 60 Mb-large scaffold 3. Note the strict enrichment of CENH3 on the Chama satellite array. E Cha. luteum interphase and metaphase chromosomes show colocalization of anti-CENH3 (magenta) and Chama repeat-specific (green) immunoFISH signals. F Chama satellite repeats are located in the knob-like structures of pachytene chromosomes. G The immunolabelling of CENH3 (magenta) in monocentric Sorghum bicolor. The chromosomal constriction in the enlargement is indicated by an arrowhead. Chromosomes were counterstained with DAPI. B, E–G At least two independent experiments were carried out to confirm the reproducibility of the labeling patterns. C Source data are provided as a Source Data file.
