Fig. 1: Cryo-EM structure of the IL-17RA/IL-17RB/ACT1 complex.
From: Structural basis for ACT1 oligomerization induced by IL-17 receptor hetero-tetramer
a Schematic domain organization of IL-17RA, IL-17RB, and ACT1. b Expression and purification of the complex between the IL-17RB-RA fusion and ACT1. The upper panel shows the constructs for protein expression. The lower left and middle panels show the gel filtration chromatography profile and SDS-PAGE gel analyses of the complex. The peak fraction highlighted by the red box was used as the cryo-EM sample. The results shown are representatives of at least three repeats. A sample image of the IL-17RA/RB/ACT1 filaments is shown in the lower right panel. c Cryo-EM map (left) and model (right) of the IL-17RA/RB/ACT1 complex. d Cartoon representation of the structure of the IL-17RA/RB/ACT1 complex.
