Fig. 5: IHx increases fatty acid oxidation and alters fatty acid composition at P17. | Nature Communications

Fig. 5: IHx increases fatty acid oxidation and alters fatty acid composition at P17.

From: Dysregulated hippocampal fatty acid metabolism following intermittent hypoxemia-induced neonatal brain injury is rescued by treatment with acetate

Fig. 5: IHx increases fatty acid oxidation and alters fatty acid composition at P17.The alternative text for this image may have been generated using AI.

a SeahorseXF Mito Fuel Flex test performed in single cell suspensions from Nx and IHx hippocampus at postnatal day (P) 11, P17, and P30 (P11 n = 7/group; P17 and P30 n = 3/group; biological replicates). Glc glucose, Gln glutamine, FA fatty acid. b Ex-vivo hippocampal [1-14C]-oleic fatty acid oxidation to 14CO2 in Nx and IHx mice (P11 n = 9/group; P17 n = 8/group; P22 Nx n = 14, IHx n = 13; P30 Nx n = 8, IHx n = 9; biological replicates). c Ex-vivo hippocampal [1-14C]-oleic fatty acid oxidation to 14CO2 in control and CPT2 astrocyte-specific knockout (CPT2G−/−) P17 mice that underwent Nx or IHx (control Nx n = 9, IHx n = 15; CPT2G−/− Nx n = 11, IHx n = 16; biological replicates). d-i Total fatty acids quantified by gas chromatography with tandem mass spectrometry (GC/MS) in (d, f, h) male and (e, g, i) female Nx and IHx hippocampus at P17 (Male: Nx n = 5, IHx n = 6; female: Nx n = 6, IHx n = 7; biological replicates). d, e Data are presented as a heatmap of % fatty acids from total fatty acids z-scores. Content of (f, g) total and (h, i) ≥20 carbon-long saturated, ω-9, ω-5-7, ω-6 and ω-3 fatty acids. For (f–i) data points (circles/triangles) represent ug of FA/total lipid and bars represent % of total fatty acids. Black * is significantly different as ug of FA/total lipid and grey * as % of total fatty acids. (a–c, f–i) data are presented as mean ± SEM. Statistical analysis for (a) by 2-tailed unpaired student’s t-test, for (b–i) by unpaired Mann-Whitney 2-tailed test; *p < 0.05 Nx vs IHx within the same age (For (a) P11 Glc p = <0.000001, FA p = 0.0004, P17 FA p = 0.0075, P30 FA p = 0.0241, for (b) P11 p = 4.10×10-5, P17 p = 0.0002, for (c) Nx control vs IHx control p = <0.0001, IHx control vs IHx CPT2G−/− p = <0.0001, for (g) ω-9/Total Lipid (ug) p = 0.0140, ω-9/Total FA (%) p = 0.0012, ω-5-7/Total FA (%) p = 0.0350, for (h) ≥20 C ω-9/Total FA (%) p = 0.0043, ≥20 C ω-5-7/Total FA (%) p = 0.0303, and for (i) ≥20 C Saturated/Total Lipid (ug) p = 0.0012, ≥20 C Saturated/Total FA (%) p = 0.0023, ≥20 C ω-9/Total Lipid (ug) p = 0.0012, ≥20 C ω-9/Total FA (%) p = 0.0012, ≥20 C ω-5-7/Total FA (%) p = 0.0082). FA fatty acids, DPM disintegrating parts per million, CPT2 carnitine palmitoyltransferase 2, n.d. not detected, Sat saturated. Source data are provided as a Source Data file.

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