Fig. 2: 4-D model of forebrain growth.
From: Resolving forebrain developmental organisation by analysis of differential growth patterns
A Stills from 4-D reconstruction of HH10-HH20 fate maps. Colours show prospective forebrain regions, and their topological relationships, at HH10 and HH20. B ‘Digital dye’ applied at HH10 (ventrolateral and dorsolateral views shown to aid visualisation) with resulting growth patterns, and comparison to in vivo examples. C Simplified growth patterns (upper), and morphology resulting from isometric (circles) and directional (lines) growth (lower). D ‘Digital dye’ spots progress to growth lines connecting eye and forebrain; oblique anterior-ventral views. E HCR-stained neuroectoderms, ventral views (n = 3-4 per stage). F Model depicted as simplified fate map with dark lines and asterisks tracking position of thalamus and posterior hypothalamic cells relative to the ZLI. Lines intersect at a ‘hinge’-like point. G Movement of ‘digital dye’ spots (green, red; arrows) in relation to HH10 ventral inflection point (upper, asterisk) and HH20 cephalic flexure (lower, asterisk). Hypo hypothalamus, ABB alar-basal boundary, ZLI zona limitans intrathalamica. Scale bars − 250 μm.
