Fig. 4: Growth patterns may distort the dorsoventral axis in mouse.
From: Resolving forebrain developmental organisation by analysis of differential growth patterns
A, B HCR-stained E11 mouse brains. Rectangles - positions shown in detail. B lower right: Foxa1+ve ventral midline domains, including Arx+ve floor plate (n = 3). C Proposed dorsoventral (D-V) axis, E11 mouse. D, E, G) HCR-stained E8-E13 mouse brains (n = 2 E8, n = 3 each, E9-13). Dotted lines in (E, G) indicate position of ZLI (white asterisks), and connect base of ZLI to anterior limit of Pitx2+ve ventral midline (*A in (E)) or Shh/Foxa1+ve ventral midline (coloured asterisks in (G)). *P = ventral midline meeting point of Pitx2+ve posterior limit and Arx+ve floor plate anterior limit. F Angles made between ZLI and lines from base of ZLI to position *A (as per insets in E) or position *P, at E11 and E13. Mean ± standard deviation. n = 8 (E11), 5 (E13), ****p < 0.000024, ***p = 0.000463, two-sided t test. Source data are provided as a Source Data file. H DAPI-stained E11-15 mouse forebrains, same samples as in (G). White dotted lines - outline of thalamus. Dotted lines—anterior edge of Shh (green) or Foxa1 (red) domains in ZLI and posterior hypothalamus. I Expansion of the thalamus may displace the Shh+ve ZLI, causing the angle between the ZLI and posterior hypothalamic Shh expression domain to become more acute between E13 and E15. Scale bars − 250 μm.
