Fig. 4: Structural optimization of the nascent base pair and reaction center.
From: Directed evolution of a TNA polymerase identifies independent paths to fidelity and catalysis
a Nucleic acid structural parameters plotted across generations of TNAP. Measurements reflect changes in the distance from the primer to the a-phosphate and key conformational rotations observed for the nascent base pair formed between the templating base (dT) and incoming triphosphate (dATP or tATP). b Structural comparisons of the nascent base pair and reaction center observed for Kod DNAP and generations of TNAPs. DNA and TNA are shown as stick models with the distance from the primer to the a-phosphate denoted with a dashed yellow line and numerical value. Top (c) and side (d) views of the geometry of the nascent base pair across generations of TNAPs via global structural alignment to Kod DNAP. TNAP generations are color-matched to Fig. 1.
