Fig. 6: Engineering allulose-responsive circuit for enzyme production. | Nature Communications

Fig. 6: Engineering allulose-responsive circuit for enzyme production.

From: Computational design of allulose-responsive biosensor toolbox for auto-inducible protein expression and CRISPRi mediated dynamic metabolic regulation

Fig. 6: Engineering allulose-responsive circuit for enzyme production.

a The schematic diagram of the designed allulose-induced expression system for ThMI production. b The crude enzyme activity of ThMI in CgWT-MI and CgM4-MI strains. The inducer concentration was 5 mM. c The schematic diagram of allulose-mediated autoinduction expression system (AMAE) for ThMI production. d The glucose consumption and allulose synthesis in strain AutoCgM4-MI. Also, the ThMI production in AutoCgWT-MI and AutoCgM4-MI was compared. Data are presented as mean values +/−SD (n = 3 independent experiments). Source data for (b) and (d) are provided as a Source Data file.

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