Fig. 5: MtrI binding to MtrCDE and MtrA is oxygen-dependent.
A Overview of MtrI (green) location in Mtr. MtrI is sandwiched by MtrAcyt (pink) and MtrCDE (orange, brown, yellow). B Close-up of MtrA-MtrI interaction. Interacting residues and Factor III shown as sticks and labelled. The unstructured insertion domain of MtrA is coloured purple. C, D Ratios of iBAQ values for MtrI and MtrA from Mtr complexes purified under oxic and anoxic conditions from methanol- (C) or H₂/CO₂-grown cells (D). Bars represent the mean ± SD of MtrI/MtrA ratios from n = 3 biological replicates. P-values were calculated from log₂-transformed ratios using a two-sided paired t-test. Methanol-grown cells (C): t = 8.091, df = 2, P = 0.0149, 95% CI of the mean difference = – 7.733 to – 2.364, partial η² = 0.9704. H₂/CO₂-grown cells (D): t = 9.291, df = 2, P = 0.0114, 95% CI of the mean difference = – 8.196 to – 3.008, partial η² = 0.9774. P-values are shown in the plot. E Top view of MtrI binding to the MtrCDE membrane globe (surface representation). F Side view of MtrI binding to the MtrCDE membrane globe (surface representation, clipped). G Close-up of MtrI-MtrCDE interaction. Interacting residues are shown as sticks and labelled.
