Fig. 5: Tocopherol acetate-enriched F. anhuiense K5.
From: General variation in the Fusarium wilt rhizosphere microbiome
A Growth of F. anhuiense K5 after treatment with different concentrations of tocopherol acetate for 12 h in vitro (n = 3 biologically independent samples per group). Compared with the control, the P values for the 0.1 mM and 1 mM groups were 2.72 × 10−3 and 3.00 × 10−4, respectively (two‑sided Student’s t test). B Principal coordinate analysis of the bacterial community in soil amended with tocopherol acetate (n = 5 biologically independent samples per group). C Enrichment of F. anhuiense K5 (ASV1) in soil amended with 1 mmol/kg tocopherol acetate (n = 5 biologically independent samples per group). The exact FDR‑adjusted P value for ASV1, determined using the DESeq2 package (negative‑binomial Wald test with Benjamini‑Hochberg FDR correction), was 7.07 × 10−10. D Enriched pathways of F. anhuiense K5 induced by 1 mM tocopherol acetate (n = 3 biologically independent samples per group). Pathway enrichment was assessed using a hypergeometric test. The significantly enriched pathways (P < 0.05) are shown. E, F Relative expression levels of genes related to the TonB‑dependent receptor (E) and the major facilitator superfamily transporter (F) in F. anhuiense K5 under control and tocopherol acetate treatment (n = 3 biologically independent samples per group). Compared with the control, the P values were 2.22 × 10−2 and 2.87 × 10−4, respectively (two‑sided Student’s t test). ***P < 0.001, **P < 0.01, *P < 0.05. Data in A, C, E, and F are presented as box plots showing the median (center line), the 25th and 75th percentiles (box limits), and the minimum and maximum values (whiskers). Source data are provided as a Source Data file.
