Fig. 6: Immune evaluation of candidate antigens for Mpox. | Nature Communications

Fig. 6: Immune evaluation of candidate antigens for Mpox.

From: Integrating protein language and geometric deep learning models for enhanced vaccine antigen prediction

Fig. 6: Immune evaluation of candidate antigens for Mpox.The alternative text for this image may have been generated using AI.

a Schematic of the immunization assessment process. Specific pathogen-free (SPF) female BALB/c mice (6–8 weeks old, n = 6 biologically independent mice per group) were immunized intramuscularly with 100 µL of vaccine formulations at day 0 and day 14. Created in BioRender. Zai, X. (2025) https://BioRender.com/jrjco9u. b Specific total IgG antibody levels in mouse immune serum (mean ± SEM, n = 6 biologically independent mice per group). c Specific IgG antibody subclasses in mouse immune serum (mean ± SEM, n = 6 biologically independent mice per group). d Detection of neutralizing antibodies against ectromelia virus (mean ± SEM, n = 6 biologically independent mice per group). e Protective efficacy in a lethal orthopoxvirus challenge. One-way analysis of variance (ANOVA) followed by Tukey’s post hoc test (for multiple group comparisons) and unpaired two-tailed t-test (for pairwise comparisons) were used to analyze differences between groups. Exact p-values for all comparisons are provided in the figure.

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