Fig. 5: Chemosensory neurons regulate exopher production in response to pathogen metabolite exposure. | Nature Communications

Fig. 5: Chemosensory neurons regulate exopher production in response to pathogen metabolite exposure.

From: Volatile and non-volatile pathogen cues shape host extracellular vesicles production in pre-infection response

Fig. 5

a Exopher production upon exposure to PA14 volatile metabolites is dependent on ASK, ADL, and AWC neurons (n = 90, 90, 90, 88, 90, 88, 90 and 90 worms (for respective columns), N = 3 independent experiments; n = 80, 80, 90, 90, 90, 90, 90 and 90 worms (for respective columns), N = 3 independent experiments; n = 110, 120, 80, 90, 90 and 90 worms (for respective columns), N = 3-4 independent experiments). The data are presented in three separate graphs because three different exopher reporter lines, each exhibiting varying levels of basal exopher production, were used. The usage of different exopher reporter lines was due to crossing incompatibilities with specific neuronal ablation mutants. b Regulation of exopher production upon PA14 non-volatile secretome exposure involves multiple neurons, including ASK, ADL, ASI, AWB, AWC, and ASH (n = 90, 90, 90, 90, 90, 90, 120 and 120 worms (for respective columns), N = 3-4 independent experiments; n = 120, 120, 90, 90, 90, 90, 90 and 90 worms (for respective columns), N = 3-4 independent experiments; n = 90 worms (for each column), N = 3 independent experiments). The data are presented in three separate graphs because three different exopher reporter lines, each exhibiting varying levels of basal exopher production, were used. The usage of different exopher reporter lines was due to crossing incompatibilities with specific neuronal ablation mutants. c Summary of experimental results: A table summarizing the experimental findings, including p-values. Created in BioRender. Kolodziejska, K. (https://BioRender.com/snvoju0). Data information: Differences in basal exopher levels observed among wild-type controls are associated with the use of different transgenes for exopher visualization, as detailed in Supplementary Data 3. Data are presented as medians with interquartile ranges. Statistical analyses were performed using the two-tailed Mann-Whitney test, with non-significant p values (p > 0.05) in pink and significant p values (p <0.05) in blue.

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