Fig. 1: 3D MINFLUX images of RyR2 sdAB labeling in HEK293 cells.

A. x-y overview of anti-GFP sdAB labeled RyR2s in a HEK293 cell stably expressing RyR2D4365-GFP. B. The corresponding x-z view illustrates the complex 3D distribution of the localizations. C. A small region (dashed rectangle in A), shows that localizations that appear adjacent in an x-y view extend over considerable distances along the z-axis (D). Color indicates z-elevation. Scale bars A, B: 500 nm; C, D: 100 nm.