Fig. 2: Endothelial cell shrinkage and rearrangement underlie vessel constriction and elongation. | Nature Communications

Fig. 2: Endothelial cell shrinkage and rearrangement underlie vessel constriction and elongation.

From: Circumferential actomyosin bundles anchored by CCM1 drive endothelial cell contraction and vessel constriction

Fig. 2: Endothelial cell shrinkage and rearrangement underlie vessel constriction and elongation.

a Schematic illustration of radial and axial axes in an unwrapped EC. b Cell area in aECs and vECs from 2-4 dpf (aEC: n = 12/13/24 cells; vEC: n = 12/25/21 cells at 2/3/4 dpf; from 12/16/17 embryos at 2/3/4 dpf, 4 experiments). Statistical significance was assessed by one-way ANOVA with Tukey’s multiple comparisons test. Data are shown as mean ± SD. c, d Strain analysis at radial (c) and axial (d) axes in aISVs between 2 and 4 dpf. Yellow bars, cell number strain; red bars, cell strain; grey bars, vessel strain. Values are indicated above/below each bar. e, f Strain analysis at radial (e) and axial (f) axes in vISVs between 2 and 4 dpf. Yellow bars, cell number strain; blue bars, cell strain; grey bars, vessel strain. Values are indicated above/below each bar. g Schematics summarising aISV and vISV remodelling between 2 and 4 dpf, highlighting contributions of cell shrinkage and rearrangement. Shorten: cell length decreases at axial axis; Narrow: cell width decreases at radial axis; Shrink: reduces in both axial and radial axes. Source data are provided as a Source Data file.

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