Fig. 4: Endothelial cells exhibit cortical actin remodelling, with circumferential actin formation correlating with vessel constriction. | Nature Communications

Fig. 4: Endothelial cells exhibit cortical actin remodelling, with circumferential actin formation correlating with vessel constriction.

From: Circumferential actomyosin bundles anchored by CCM1 drive endothelial cell contraction and vessel constriction

Fig. 4: Endothelial cells exhibit cortical actin remodelling, with circumferential actin formation correlating with vessel constriction.

a Representative images of circumferential, mesh, longitudinal and mixed actin organisations at 2 dpf. Scale bars: 10 μm (left), 5 μm (middle). b Percentage of each actin organisation from 2 to 4 dpf in aISVs and vISVs. Total number of ISVs (from 22/25/28 embryos at 2/3/4 dpf, 5 experiments) is indicated on the top of the bar. c–e Still frames from time-lapse movies (Supplementary Movies 5–7) at 2 dpf illustrating circumferential actin anchored at cell membrane (c aISV), connected to cell-cell junctions (d vISV), or linked to both the membrane and cell junctions (e aISV). In the schematics, circumferential actin is highlighted in red. Black lines trace the cell boundary at the current time point, and grey serrated lines represent the outline of the cell membrane or cell-cell junctions at the first time frame. Scale bar, 5 μm. f Kymograph of the yellow ROI in (e) and measurements of diameter over time. ISV intersegmental vessel, aISV arterial ISV, vISV venous ISV. Source data are provided as a Source Data file.

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