Fig. 2: Stabilizing PCB production in stationary phase.
From: A stationary phase-specific bacterial green light sensor for enhancing metabolite production
a Schematic of plasmid- (top), initial chromosomal- (center), and optimized chromosomal (bottom) ho1-pcyA expression cassettes. Red indicates a genetic modification introduced relative to the cassette above. b PCR amplification of the three ho1-pcyA cassettes from E. coli cultures grown to exponential (E) or stationary (S) phase. Expected size is 1.7 kb. n = 2 replicates on 2 separate days were collected. Unmodified gel images and replicate gels are provided in Supplementary Fig. 2. c PCB levels produced from each cassette in exponential and stationary phases as reported by Cph1(Y176H):PCB far-red fluorescence. Individual (n = 3) replicates on 3 separate days are shown. Bars represent the mean across the three replicates. d Growth curves of parent BW29655 (WT) and different PCB producing strains (plasmid or chromosomal (chr), black). OD600, sat indicates the carrying capacity, or maximum OD600 reached, by cultures of the engineered strain. Shaded region represents the standard deviation of the mean of n = 3 replicate experiments performed on 3 separate days. Source data are provided as a Source data file.
