Fig. 4: Using static light signals to optimize p-coumaric acid production in stationary phase.
From: A stationary phase-specific bacterial green light sensor for enhancing metabolite production
a Diagram of the engineered p-CA biosynthetic pathway, 24-well plate batch culture fermentation strategy, and HPLC detection method. b CcaSRstat:tal-sfgfp strain, wherein green light induces tal-sfgfp expression. c Relationship between green light intensity and tal-sfgfp expression for CcaSRstat:tal-sfgfp in stationary phase. Error bars represent the standard deviation of the mean of 3 replicates collected on the same day. d Light response of control tal-sfgfp strain lacking CcaSRstat. Bars represent the arithmetic mean of three biologically independent replicates collected on the same day. e CcaSRstat:tal strain, which is used for p-CA production. f Relationship between green light intensity and p-CA titer. Error bars represent the standard deviation of the mean of thre independent biological replicates collected on the same day. Black lines represent Hill function fits (Methods). K is the green light intensity resulting in half maximal activation of p-CA production. n represents the Hill coefficient of the fit. g Control experiments measuring p-CA levels produced by the non-engineered (WT) and tal strain lacking CcaSRstat. Bars represent the arithmetic mean of three independent biological replicates collected on the same day. Source data are provided as a Source data file.
