Fig. 2: The investigation of T-Expand interacting with Jurkat cells.
From: Dextran-based T-cell expansion nanoparticles for manufacturing CAR T cells with augmented efficacy
a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or naked NPs both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d, The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real-time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or DynabeadsTM, scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.
