Fig. 1: Deep mutational scanning (DMS) of FDX1. | Nature Communications

Fig. 1: Deep mutational scanning (DMS) of FDX1.

From: Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis

Fig. 1: Deep mutational scanning (DMS) of FDX1.

A Two functions of FDX1: (1) reducing elesclomol (ES)-bound Cu(II) to Cu(I), which facilitates its release and subsequent toxicity; and (2) enabling LIAS mediated lipoylation. B Viability of parental, FDX1 KO, or FDX1 KO with FDX1 reconstituted ABC1 cells 72 h after treatment with ES. Viability data is presented as mean ± SD of four biological replicates. Immunoblots of indicated cell lines are provided in Fig. 3D. C DMS screen experimental design. mFDX1 denotes mutant FDX1. D Normalized distributions of ABC1 or HEK293T cells harboring missense, nonsense, or silent mutations of FDX1. LOG2FC denotes log2 fold change in viability.

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