Fig. 3: Charge flip on residues D136 and D139 abolishes FDX1 mediated cuproptosis and protein lipoylation in cells. | Nature Communications

Fig. 3: Charge flip on residues D136 and D139 abolishes FDX1 mediated cuproptosis and protein lipoylation in cells.

From: Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis

Fig. 3

A Viability of the indicated cell lines 72 h after treatment with ES. B, C Proliferation (represented as the number of replications) of the indicated cell lines were measured over the indicated times using live cell imaging. D, E Cell lysates obtained from the indicated cell lines were subjected to immunoblotting and assessed with the indicated antibodies shown on the left. F Proliferation (represented as the percentage of confluence) of the indicated HEK293T cell lines were measured over the indicated times in varying glucose conditions using live cell imaging. In (A–C) and (F), data is presented as mean ± SD of three (A), eight (B), eight (C), and three (F) biological replicates.

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