Fig. 2: The lymphatic vessels were expanded after tendon injury. | Nature Communications

Fig. 2: The lymphatic vessels were expanded after tendon injury.

From: Lymphatic–stem cell crosstalk promotes tendon regeneration via Notch1–Srebp2–mediated cholesterol metabolism

Fig. 2: The lymphatic vessels were expanded after tendon injury.The alternative text for this image may have been generated using AI.

A, B The lymphatic vessels labeled by PROX1 in Achilles tendon and Patellar tendon using Prox1-CreERT2; tdTomato mice, as presented by projection of 3D images after TESOS clearing. C The co-immunolabelling of Prox1-CreERT2; tdTomato positive lymphatic vessels and CD31 in neonatal and adult Achilles tendon with or without tendon injury after 7 days, assessed by projection of 3D images after TESOS clearing. D The semi-quantitative analysis of tdTomato positive lymphatic vessels density in the indicated groups. E The top 5 significant signaling pathways based on the different genes between the control and injury groups. Those shown in red were higher enriched in the injury sample. F The violin plots showed the increased 5 genes expression after injury in TSCs, TPCs, tenocytes, LECs and VECs. G The expression of Reelin in neonatal and adult tendons with or without tendon injury, presented by immunofluorescence staining in sectioned tendon tissue from Prox1-CreERT2; tdTomato mice. H The expression of Reelin and LYVE-1 in neonatal and adult tendons with or without tendon injury. White triangle: lymphatic vessels; Yellow triangle: lymphatic valves. Each dot represents one individual replicate (n = 5 biological replicates per group in D). Statistical significance was analyzed by unpaired T test between control and injury groups at different ages, respectively.

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