Fig. 1: 5′ RACE analysis of VACV transcripts.
From: Vaccinia virus mRNAs containing long 5′-poly(A)-leaders lack a canonical 5′-methylguanosine cap
A H5R early gene transcripts are all capped (see also Supplementary Fig. S1); the first 10 nucleotides of the 5′ untranslated region are displayed; the ATG translation initiation start codon is located 78 nt downstream from the TSS (Supplementary Fig. S1). B Only a part of the G8R intermediate transcript contains 5′ cap moieties (see also Supplementary Fig. S2). C All A17L late transcripts contain long 5′ poly(A) leaders and are not capped (see also Supplementary Fig. S3). The upper sequence in each panel corresponds to the viral template DNA. The TSS (black arrow) and INR (underlined) were annotated according to Yang et al. 2011 and 201216,37. The sequences depicted below the template DNA represent individual sequenced cDNA clones. The 5′ untranslated regions are shown up to the ATG translation start codon, except in panel (A). Nucleotides identical to the viral template DNA are labeled in red. Nucleotides added in a nontemplated manner are labeled in green. The guanosine residues corresponding to the 5′ mRNA cap are marked in black. All sequences are shown in the 5′-to-3′ orientation, regardless of their transcriptional orientation in the VACV genome.
