Fig. 8: A side-by-side comparison of the 5′ poly(A) leader length and the occurrence of the m7G cap in the VACV transcripts of the wild-type and vD9muD10mu strains.
From: Vaccinia virus mRNAs containing long 5′-poly(A)-leaders lack a canonical 5′-methylguanosine cap
The plot shows a comparison of transcripts purified from HeLa cells infected with the VACV WR strain and transcripts purified from BHK-21 cells infected with the vD9muD10mu double mutant (marked as dd). Similarly as in Fig. 2, data from both VACV strains show a negative correlation between the 5′ poly(A) leader length (left Y-axis) and cap occurrence (right Y-axis) in mRNAs from different successive GTCs represented by selected early, intermediate and late vaccinia virus genes (X-axis). The box-whisker plot (in gray) represents the lengths of nontemplated 5′ poly(A) leaders. The lower bar, upper bar, bottom and top of the boxes represent the 10th percentile, 90th percentile and the first and third quartiles, respectively. The square dot and inner line in each box represent the mean and median, respectively. The proportions of 5′ capped transcripts [%] among all mRNAs tested for each gene are shown in red. In total, 359 individual cDNAs were used for this analysis (Supplementary Figs. S1–S3 and Supplementary Table S2).
