Fig. 1: Expression of CYP2J2 and sEH and lipid profiles during inflammation in human skin tissue.

Paraffin-embedded skin punch biopsies were obtained at baseline, 4, 8 and 24 h post UVKEc injection. Sections were incubated with A CYP2J2 and B sEH primary antibodies and visualised by avidin-biotin complex-based detection methods. The sections were subsequently counterstained with haematoxylin. C Skin biopsies at baseline, 4, 8 and 24 h were stained and the % area of staining quantified for (i) CYP2J2, (ii) Pan CYP2C, (iii) sEH, (iv) mEH (n = 1–3; biologically independent samples). D Healthy volunteers received an intradermal injection into the forearm of UV-killed E. coli (UV-KEc), resulting in a local inflammatory response. Local inflammatory exudate was collected at baseline, 4, 8, 14, 24, 48 and 72 h post UVKEc injection and analysed by mass spectrometry for lipidomic profiles. (i) Levels of polyunsaturated fatty acids (PUFAs) were quantified in pg/ml (n = 2–5; biologically independent samples). Cytochrome P450 products and Epoxide Hydrolase products (ii) 14,15-DHET, (iii) 12,13-DiHOME, (iv) 19,20-DiHDPA and (v) 17,18-DiHETE were quantified during inflammation in pg/ml (n = 4-5; biologically independent samples). (vi) 12,13-EpOME was quantified in pg/ml (n = 4-5; biologically independent samples). The dotted line represents baseline levels of the lipids. (vii) Total CYP450 profiles (viii) total COX products were normalised relative to baseline levels to show the changing profile during acute and resolving inflammation (each dot is representative of 4–5 biologically independent samples). Data were assessed for normalisation using the D’Agostino & Pearson test, the Shapiro–Wilk test and visualised using a QQ plot. Data are presented as mean ± SD. Source data are provided as a Source Data file.