Fig. 7: CD4 T cells are reduced at the inflammatory site with therapeutic sEH inhibition.

The forearms of participants were intradermally injected with UV-killed E. coli (UV-KEc). Two hours prior to (prophylactic) or 4 h after (therapeutic) UV-KEc injection, participants were dosed with 15 mg of GSK2256294. Local inflammatory exudate was subject to flow cytometric analysis at 4 and 24 h (prophylactic) and 24 and 48 h (therapeutic). A (i) CD4, (ii) CD4 T-regulatory, (iii) CD8 T cell numbers per blister at 48 h (therapeutic) (untreated: n = 5; GSK2256294: n = 5; biologically independent samples). (iv) % dead T cells at 24 and 48 h (therapeutic) (untreated: n = 6-7; GSK2256294: n = 6; biologically independent samples). B Concentration of IL-1α in pg/ml in blister fluid at 24 and 48 h (therapeutic) (untreated: n = 10–11; GSK2256294: n = 6–12; biologically independent samples). C Monocyte subset numbers/blister at 4, 24 and 48 h in untreated participants (n = 11–18; biologically independent samples). D Time course of CD4 and CD8 subset numbers/blister in untreated participants (n = 6; biologically independent samples). E Intermediate monocytes were co-cultured with CD4 T cells at a 5:1 (T cell:monocyte) ratio for 48 h and analysed by spectral flow cytometry. The % expression on total CD4 cells was visualised for (i) CD25, (ii) CD39, (iii) CD69, (iv) CD103, (v) CLA, (vi) CTLA-4, (vii) HLA-DR, (viii) Ki67, (ix) CD45RO and (x) FOXP3+CD25+CD127− (n = 3; technical repeat). F Classical, intermediate and non-classical monocytes and CD8 T cells were co-cultured for 4 days at a 5:1 (T cell:monocyte) ratio, after which a cytotoxicity assay against K562 cells was performed (n = 2; biologically independent samples). Normality was assessed using the D’Agostino & Pearson test, the Shapiro–Wilk test and visualised using a QQ plot. Box-and-whisker plots show the median (centre line), the interquartile range (25th–75th percentiles; box), and the full data range (whiskers, minimum to maximum). Parametric data are presented as mean ± SD. Non-parametric data are presented as median ± 95% CI. Data in (A(i-iii)) were analysed using a two-tailed, Mann–Whitney t-test. Data in (A(iv), B) were analysed using two-way ANOVA mixed effect analysis with Uncorrected Fisher’s LSD. Data in (C) were analysed using two-way ANOVA mixed effect analysis with Šídák multiple comparison test. Data in (E) were analysed using a two-tailed, parametric, paired t-test. Source data are provided as a Source Data file.