Fig. 4: MAGPIE co-registration of spatial multi-omics datasets from AZX-treated rat lung. | Nature Communications

Fig. 4: MAGPIE co-registration of spatial multi-omics datasets from AZX-treated rat lung.

From: Spatially resolved integrative analysis of transcriptomic and metabolomic changes in tissue injury studies

Fig. 4: MAGPIE co-registration of spatial multi-omics datasets from AZX-treated rat lung.

a Outline of experimental design and Visium and DESI MSI data generation from a rat lung exposed to the AZX compound. b Spatial cross-correlation assessment of overlap between MSI AZX compound distribution (m/z 550.15724) and Visium NMF factors. The colour of bars reflects the spatial cross-correlation. c Spatial distribution of MSI AZX intensity distribution (capped at 99th percentile) and the scaled (0-1) Visium NMF factor activities for Factors 14, 8, and 4 (also capped at 99th percentile), shown through colour overlayed on H&E images. Only one sample was analysed for illustration. Scale bars reflect 500 μm. d Enrichment analysis on the top 100 contributing genes for NMF Factor 14, showing the Gene Ontology (GO) enriched terms. Enrichment analysis was performed using a rank-based cumulative enrichment test as implemented in g:profiler75 with g:SCS (Set Counts and Sizes) multiple testing correction. Colours represent gene intersection size between queried genes and genes annotated to each term; dot size corresponds to the recall (ratio between gene intersection size and number of term genes). Source data are provided as a Source Data file.

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