Fig. 3: The CL-mPFC pathway exerts strong feedforward inhibitory effects. | Nature Communications

Fig. 3: The CL-mPFC pathway exerts strong feedforward inhibitory effects.

From: The interconnected wirings between medial prefrontal cortex and claustrum and their governing roles in the methamphetamine reward memory in male mice

Fig. 3: The CL-mPFC pathway exerts strong feedforward inhibitory effects.

a Schematic diagram of the mPFCGlu neurons under patch clamp recording. b Representative traces of the amplitude showing the oEPSCs and oIPSCs in mPFCGlu neurons. c Representative quantification of the amplitude showing the oEPSCs and oIPSCs in mPFCGlu neurons. The amplitude of oEPSCs, two-tailed paired t-test, n = 6 cells from 3 mice, TTX, t(5) = 7.3000, p = 0.0008 vs. ACSF; TTX + 4-AP, t(5) = 7.3840, p = 0.0007 vs. TTX. The amplitude of oIPSCs, two-tailed paired t-test, n = 6 cells from 3 mice, TTX, t(5) = 8.1740, p = 0.0004 vs. ACSF; TTX + 4-AP, t(5) = 0.7363, p = 0.4946 vs. TTX. d Same as (b). e Same as (c). The amplitude of oEPSCs, two-tailed paired t-test, n = 6 cells from 4 mice, PTX, t(5) = 1.7700, p = 0.1370 vs. ACSF; NBQX + AP5, t(5) = 11.2400, p < 0.0001 vs. PTX. The amplitude of oIPSCs, two-tailed paired t-test, n = 6 cells from 4 mice, PTX, t(5) = 5.5510, p = 0.0026 vs. ACSF; NBQX + AP5, t(5) = 0.6732, p = 0.5307 vs. PTX. f Same as (a) but in mPFCGABA neurons. g Same as (b) but of oEPSCs in mPFCGABA neurons. h Same as (c) but of oEPSCs in mPFCGABA neurons. Two-tailed paired t-test, n = 6 cells from 4 mice, TTX, t(5) = 9.6090, p = 0.0002 vs. ACSF; TTX + 4-AP, t(5) = 6.7430, p = 0.0011 vs. TTX; TTX + 4-AP + NBQX + AP5, t(5) = 7.4510, p = 0.0007 vs. TTX + 4-AP. i Same as (a). j Same as (b) but of oIPSCs in mPFCGlu neurons. k Same as (c) but of oIPSCs in mPFCGlu neurons. Two-tailed paired t-test, n = 6 cells from 4 mice, TTX, t(5) = 11.0100, p = 0.0001 vs. ACSF; TTX + 4-AP, t(5) = 12.8300, p < 0.0001 vs. TTX; TTX + 4-AP + PTX, t(5) = 9.3460, p = 0.0002 vs. TTX + 4-AP. l There are four independent pathways between mPFC and CL of the two cerebral hemispheres, which are connected anterogradely to form an unclosed circuit. m In either top-down mPFC-CL pathway or bottom-up CL-mPFC pathway, there exist direct excitatory pathways and indirect inhibitory pathways between mPFCGlu neurons and CLGlu neurons. All statistical tests are two-sided. Data presented as mean ± SEM. N.S., p > 0.05, **p < 0.01. Source data are provided as a Source Data File.

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