Fig. 5: The mPFC^Glu and CL^Glu neurons are activated, while the FFI in mPFC-CL circuits is weakened under METH CPP.

a Experimental design and timeline. b Analysis of CPP score (two-way ANOVA, n = 12, F_{(1, 44)} = 14.2400, p = 0.0005) and ΔCPP score (two-tailed unpaired t-test, n = 12, t₍₂₂₎ = 6.9100, p < 0.0001). c Sample traces for the number of AP following current injection in mPFC. d The number of AP of mPFC neurons under whole-cell current-clamp configuration (two-way ANOVA, n = 6 cells from 4 mice, F_{(10, 110)} = 7.1470, p < 0.0001). e Same as (c) but in CL. f Same as (d) but in CL (two-way ANOVA, n = 6 cells from 4 mice, F_{(10, 110)} = 8.4080, p < 0.0001). g Schematic diagram of virus injection in the mPFC-CL pathway. h Representative traces showing the oEPSCs and oIPSCs in CL^Glu neurons. i Quantification of the amplitude showing the oEPSCs and oIPSCs in CL^Glu neurons. The amplitude of oEPSCs, two-tailed unpaired t-test, n = 6 cells from 4 mice, t₍₁₀₎ = 5.0150, p = 0.0005. The amplitude of oIPSCs, two-tailed unpaired t-test, n = 6 cells from 4 mice, t₍₁₀₎ = 4.2780, p = 0.0016. j Same as (g) but in the CL-mPFC pathway. k Same as (h) but in mPFC^Glu neurons. l Same as (i) but in mPFC^Glu neurons. The amplitude of oEPSCs, two-tailed unpaired t-test, n = 6 cells from 3 mice, t₍₁₀₎ = 4.1930, p = 0.0018. The amplitude of oIPSCs, two-tailed unpaired t-test, n = 6 cells from 4 mice, t₍₁₀₎ = 5.6360, p = 0.0002. m, n In the indirect pathway of the mPFC-CL interconnected circuit, the inhibitory effects of GABAergic interneurons on downstream Glu neurons were uniformly weakened in METH group mice after CPP test, leading to synchronous enhancement of mPFC^Glu and CL^Glu neuronal activities. All statistical tests are two-sided. Data presented as mean ± SEM. N.S., p > 0.05, **p < 0.01. Source data are provided as a Source Data File.