Fig. 2: The HFD reduces the activity of LSGABA neurons during feeding.
From: High-fat diet disrupts a septal control on feeding to promote obesity in male mice
a Representative image of the viral expression and position of the GRIN lens in the LS. Scale bar, 500 µm. This representative result was confirmed in 9 independent mice. b Heatmaps of the LSGABA neurons responses to Chow or HFD. Each row represents the mean activities of one neuron. c Sankey diagram showing the response ratio of LSGABA neurons to chow and HFD. d Left: example support vector machine (SVM) decoding using neuronal population activities in response to Chow and HFD. Right: performance of decoding using actual neuronal responses to chow and HFD or using neuronal responses shuffled across trial types (p < 0.05). e Schedule of the miniscope imaging before (Day 1) and after (Day 30) 4 weeks chow (Group I: Chow) or HFD (Group II: HFD) feeding. f Heatmaps showing calcium signals at Day 1 and Day 30. The black vertical dashed lines indicate the initiation of feeding behavior. g Average traces (left) and quantification of peak (right) of the activated neurons at Day 1 in Group I: Chow and Group II: HFD. h, i Average traces (left) and quantification of peak (right) of the activated (h) and inhibited (i) neurons at Day 1 and Day 30 in Group I: Chow (activated neurons: p = 0.45; inhibited neurons: p = 0.78). j, k Average traces (left) and quantification of peak (right) of the activated (j) and inhibited (k) neurons at Day 1 and Day 30 in Group II: HFD (activated neurons: p < 0.001; inhibited neurons: p = 0.75). Statistics: (d) two-tailed paired t-test, (h-k) two-sided Mann-Whitney U-test, with detailed statistics provided in Supplementary Data 1. Sample sizes are indicated in the figures. ns, no significant difference, * p < 0.05, ***p < 0.001. Data are presented as mean ± SEM. All tests are two-sided. Source data are provided as a Source Data file.
