Fig. 5: PDGFRα and THBD expression facilitate productive infection in primary monocytes. | Nature Communications

Fig. 5: PDGFRα and THBD expression facilitate productive infection in primary monocytes.

From: Viral entry shapes HCMV latency establishment

Fig. 5: PDGFRα and THBD expression facilitate productive infection in primary monocytes.

a Quantification of the number of viral particles, using HCMV-UL32-GFP, within the cytoplasm of infected THP1 monocytes with induced expression of mCherry as a control, NRP2 or THBD at 1 hpi (n = 50, 67, and 63, respectively). Viral particles were counted using FIJI image processing and statistical analysis was performed using Poisson regression. Source data are provided as a Source data file. b Flow cytometry analysis of THP1 monocytes overexpressing control, THBD and NRP2, using a dox-inducible system, infected with HCMV-GFP at 3 dpi. The gate marks the productive, GFP-bright cell population. c, e Flow cytometry analysis of PDGFRα surface expression on primary monocytes transfected with control, PDGFRα (c) and THBD (e) mRNA at 12 h after transfection. d, f Flow cytometry analysis of primary monocytes transfected with PDGFRα (d), THBD (f) or control mRNA for 12 h before HCMV infection. Cells were analyzed at 3 dpi. Gating strategies for Fig. 5b–f are shown in Fig. S1a.

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