Fig. 7: HCMV entry into macrophages is mediated through ITGB3.

a ITGB3 and ITGB1 expression in primary, THP1 and Kasumi-3 monocytes and macrophages as measured by RNA-seq. RPM, reads per million. n = 2. b, c Flow cytometry analysis of integrin β1 (b) and integrin β3 (c) cell surface levels in primary and THP1 monocytes and macrophages. d Flow cytometry analysis of control, ITGB3, ITGB1 and ITGB3 + ITGB1 knockout (KO) in THP1 macrophages infected with HCMV-GFP. Cells were analyzed at 3 dpi, p-value was calculated using a two-sided Student t test. n = 3. e, f Representative microscopy images (e) and quantification of viral particles using FIJI analysis software (f) of THP1 macrophages with ITGB3 knockout (KO) versus control knockout (n = 67 and n = 60, respectively) infected with HCMV-UL32-GFP and imaged at 1 hpi Actin staining was used to visualize the cells’ borders and DAPI for nuclei staining. Statistics was performed using Poisson regression. g Flow cytometry analysis of infected THP1 monocytes overexpressing ITGB3 and ITGAV (αvβ3), compared to mCherry control. Overexpression was induced for 24 h using doxycycline prior to HCMV-GFP infection. Cells were analyzed at 3 dpi. Gating strategies for Fig. 7b, c, d, g are shown in Fig. S1a. Source data for Fig. 7a, d, f are provided as a Source data file.