Fig. 1: DCAF10 binds acetylated N-terminal glycine residues.
From: CUL4A-DDB1-DCAF10 is an N-recognin for N-terminally acetylated Src kinases
a Schematic of peptide pull-downs combined with quantitative mass spectrometry (MS). Ac acetylation; Myr myristoylation; G glycine; x any amino acid; K lysine. Created in BioRender. Kremer, N. (2025) https://BioRender.com/fxzaxz0. b–e Volcano plots comparing binding partners of free (Nt-free) and acetylated (Nt-Ac) N-termini (aa 1–10 + KKK-biotin; Supplementary Table 1) (n = 3 independent biological replicates). b Lyn; c Src; d Fyn; e THOC7. The −log10 adjusted p-value (two-sided Student’s t test with permutation-based multiple-testing correction; y-axis) is plotted against the log2 fold change (x-axis). Threshold for significance: −log10 p-value ≥ 1.3 (p ≤ 0.05), log2 fold change ≤ −2 or ≥ 2. DCAF10 is marked in green, significant Nt-free binders in purple, and significant Nt-Ac binders in blue. f Schematic of the CUL4A-DDB1-DCAF10 E3 ligase complex including an Ac-Gly-starting substrate, the catalytic subunit RBX1, E2, and ubiquitin. Created in BioRender. Kremer, N. (2025) https://BioRender.com/0whobwz. g Volcano plot comparing Nt-Ac and myristoylated (Nt-Myr) N-termini of Lyn. The −log10 adjusted p-value (two-sided Student’s t test with permutation-based multiple-testing correction) is plotted against the log2 fold change, as in (b–e). Threshold for significance: −log10 P value ≥ 1.3 (P ≤ 0.05), log2 fold change ≤ −2 or ≥2 (n = 3 independent biological replicates). h Sequence logo of nine N-terminal sequences significantly binding to DCAF10 (including six represented in Supplementary Fig. 1).
