Fig. 2: Impact of FRQ’s NLSs on the circadian clock.

A–E Bioluminescence recordings of Neurospora frq-lucP reporter strains expressing the indicated frq alleles. Calculated period lengths (τ ± SD) and damping coefficients (γ ± SD) are shown. Results shown are from at least two independent experiments for each construct with a total of clones specified. A WT frq. The trajectory is the normalized average of 31 replicates from 4 independent clones. Also shown as dotted blue line for comparison in (B–E). B frqmNSL1. 27 replicates, 7 clones. C frqmNLS2. 15 replicates, 3 clones. D frqmNLS3. 16 replicates, 3 clones. E frqmNLS1/2/3. 40 replicates, 11 clones.