Fig. 3: Binding of saturating amounts of FRH to FRQ suppresses the formation of FRQ nuclear foci in U2OStx cells. | Nature Communications

Fig. 3: Binding of saturating amounts of FRH to FRQ suppresses the formation of FRQ nuclear foci in U2OStx cells.

From: Casein kinase 1a mediates a two-step subunit remodeling mechanism to regulate the FRQ-FRH circadian clock complex

Fig. 3: Binding of saturating amounts of FRH to FRQ suppresses the formation of FRQ nuclear foci in U2OStx cells.

The indicated mK2-tagged FRQ versions were co-expressed with FRH-mNG. Interactions are represented schematically. A Co-expression of mK2-FRQmNLS1/2/3 (50 ng plasmid DNA) and non-saturating amounts of FRH-mNG (16.5 ng). mK2-FRQmNLS1/2/3 and FRH-mNG co-localize in cytoplasmic foci. B Co-expression of mK2-FRQmNLS1/2/3 (50 ng plasmid DNA) and saturating amounts of FRH-mNG (50 ng). mK2-FRQmNLS1/2/3 and FRH-mNG are dispersed in the nucleus. C Co-expression of mK2-FRQ and non-saturating amounts of FRH-mNG. mK2-FRQ and FRH-mNG co-localize in nuclear foci. D mK2-FRQ and saturating amounts of FRH-mNG. mK2-FRQ and FRH-mNG are dispersed in the nucleus. E, F 50 ng mK2-FRQ6B2 or mK2-FRQ9 with saturating amounts FRH-mNG. mK2-FRQ6B2 and mK2-FRQ9 form nuclear foci that do not contain FRH-mNG. G mK2-FRQC-term (50 ng) and saturating amounts FRH-mNG. Both proteins are dispersed in the nucleus. Panels show representative subcellular expression patterns. Quantitative data are summarized in Supplementary Table 1.

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