Fig. 4: FRQ interacts with WC-1 and WCC in absence of FRH. | Nature Communications

Fig. 4: FRQ interacts with WC-1 and WCC in absence of FRH.

From: Casein kinase 1a mediates a two-step subunit remodeling mechanism to regulate the FRQ-FRH circadian clock complex

Fig. 4: FRQ interacts with WC-1 and WCC in absence of FRH.

A–C Co-expression of WC-1-mNG (n = 3). A with mK2-FRQ. WC-1-mNG is enriched in mK2-FRQ nuclear foci. B with mK2-FRQ6B2. WC-1-mNG is enriched in mK2-FRQ6B2 nuclear foci. C with mK2-FRQ9. WC-1-mNG does not co-localize with mK2-FRQ9 nuclear foci. D–F Co-expression of WC-1-mNG and untagged WC-2 (n = 3). D with FRQ. WC-1-mNG is enriched in mK2-FRQ nuclear foci. E with mK2-FRQ6B2. WC-1-mNG is enriched in mK2-FRQ6B2 nuclear foci. F with mK2-FRQ9. WC-1-mNG is not enriched in FRQ9-mNG nuclear foci. Lower panels: The enrichment of WC-1-mNG in foci of the indicated mK2-FRQ version over nuclear regions outside of foci was quantified. Foci from 10 cells were evaluated. Plotted is Mean +/− SD (exact Mean and SD can be found in Source Data file). One-tailed Wilcoxon signed-rank test: A and E: **p = 0.006; B and D: **p = 0.002; C and F: p = 0.3, ns not significant.

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