Fig. 6: Phosphorylation of FRQ by CK1a triggers its dissociation from FRH and nuclear export. | Nature Communications

Fig. 6: Phosphorylation of FRQ by CK1a triggers its dissociation from FRH and nuclear export.

From: Casein kinase 1a mediates a two-step subunit remodeling mechanism to regulate the FRQ-FRH circadian clock complex

Fig. 6: Phosphorylation of FRQ by CK1a triggers its dissociation from FRH and nuclear export.

A Western blot analysis of mK2-FRQ, mK2-FRQ6B2 and mK2-FRQ9 co-expressed with and without CK1a in U2OStx cells for the indicated time periods. The samples were loaded side by side on the same gel in the order shown. They were cropped into separate panels in pairs to facilitate comparison. B Widefield fluorescence microscopy with DAPI staining of cells expressing mK2-FRQ with and without untagged CK1a. U2OStx were analyzed 6 h post DOX induction (n = 2). C–E Incucyte time courses of transfected U2OStx cells upon co-expression of FRH-mNG and untagged CK1a with C mK2-FRQ, D mK2-FRQ6B2, and E mK2-FRQ9. LMB was added where indicated (n = 3; LMB: n = 2).

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