Fig. 2: rad51-ED and rad51-KN are recombination-proficient and cannot suppress the recombination defect of rad54.
From: Rad51 determines pathway usage in post-replication repair
A Direct repeat recombination assay66. HO: HO endonuclease cleavage site. B Spontaneous recombination rate data. C Recombination frequency data after DSB induction. Strains were freshly dissected from diploid strains and single spore clones were assayed, a minimum of 11–16 single clones were assayed per genotype, specific values for n are given with the genotypes. The median recombination rates/frequencies are given with the 95% confidence intervals100. The indicated p-value is derive from a two-tailed t-test. Wildtype (WDHY3383, n = 16 in B, n = 16 in C), rad52Δ (WDHY3348, n = 13, 13), rad51Δ (WDHY3915, n = 11, 11), rad51-KN (WDHY3385, n = 15, 15), rad51-KN rad54Δ (WDHY3463, n = 15, 13), rad51-ED (WDHY3386, n = 15, 14), rad51-ED rad54Δ (WDHY3462, n = 13, 13), rad54Δ (WDHY3349, n = 13, 13). The viability data are shown in Supplementary Fig. 2. The data are all relative to the number of colony forming units (cfu) on YPD. Relevant p values are given. ns: not significant. No other differences involving rad51-ED and rad51-KN were significant. Source data are provided as a Source Data file.
