Fig. 6: Rad51-KN and Rad51-ED are defective in protecting dsDNA from degradation by Exo1 or Dna2-Sgs1.

A Cartoon depicting the nuclease assay with Exo1 performed in panels B-C. The red asterisks represent the position of the radioactive label. B Representative nuclease assays of Exo1 using a 100 bp DNA substrate in the presence of increasing concentrations of Rad51 wild type and variants, as indicated. In lanes 7, 14 and 21, the substrate was boiled to indicate the position of the ssDNA. C Quantitation of experiments such as shown in (B). Averages shown; error bars, SEM; 3 independent experiments (n = 3). D Cartoon depicting the nuclease assay with Sgs1-Dna2 performed in (E, F). The red asterisks represent the position of the radioactive label. E Representative nuclease assay with Dna2-Sgs1 in the presence of 4 µM Rad51 wild type and variants. In lane 7, the substrate was boiled to indicate the position of the ssDNA. F Quantitation of experiments such as shown in E. Averages shown; error bars, SEM; 3 independent experiments (n = 4). Source data are provided as a Source Data file.