Fig. 2: PURE-seq profiles circulating tumor cell from melanoma patient blood samples.
From: PURE-seq integrates FACS and PIP-seq for single-cell genomics of ultra-rare cells
A Blood samples from metastatic melanoma patients undergoes PBMC isolation followed by CD45- cell sorting into PURE-seq reactions (n = 2 patients, 8 mL blood each). B UMAP plot of all cells (n = 896) from ctrl (CD45+) and neg (CD45−) sorted samples. C UMAP identifies 3 clusters, which include 1 T/NK cell cluster and 2 CTC-enriched (dotted line). D Violin plots of expression levels of canonical genes (T/NK, platelets, endothelial, fibroblast, melanoma) in the different clusters. E InferCNV analysis of CTCs and reference hematopoietic cells by each patient.
