Fig. 6: Freely-behaving mice achieve similar discrimination performance to optogenetic stimulation with μLED arrays as head-fixed.

a Schematic diagram of the setup used for freely-behaving experiments. b Photographs within the chamber showing the mouse moving to the infrared (IR) beam to begin the task. c Schematic illustration of freely-behaving discrimination task. d Cumulative time spent in the IR beam for each trial in the first session (left) and in the eleventh session (right) for Mouse 1. e Learning curves for each mouse. Each bar represents the average cumulative time spent in the IR beam per trial over a session/day (mean ± standard deviation), with bar colour representing trial type (right axis). The thick line shows the accuracy (% of trials correct) for each session (left axis) with the chance level indicated by the dashed line. Cortical irradiance per μLED shown above corresponding session(s). The exact number of trials per session is given in Supplementary Data 1.