Fig. 1: The SET domain of SET-24 is a member of the ScSet3 SET subfamily and is catalytically inactive.
From: A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory
a Maximum likelihood phylogenetic tree comparing the protein sequences of SET domains of selected SET domain-containing genes in C. elegans. Values next to the tree nodes are branch supports, calculated with 1000 ultrafast bootstrap replicates. b Phylogenetic tree of human SET domains and the SET domain of C. elegans SET-24. Values next to the tree nodes are branch supports, calculated with 1000 ultrafast bootstrap replicates. c Protein sequence alignment of the C. elegans SET-24 SET domain with SET domains from catalytically inactive Set3 SET subfamily and catalytically active CeSET-25, ScSET1, HsMLL1, and HsMLL3. The residues important for catalytic activity are highlighted. Ce, Caenorhabditis elegans; Hs, Homo sapiens; Sc, Saccharomyces cerevisiae. d Schematic representation of the bioluminescent assay to measure methyltransferase activity. e Bar graph showing the in vitro methyltransferase activity of recombinant MBP-ScSET2 (SET2 from Saccharomyces cerevisiae) and MBP-CeSET-24 (SET-24 from Caenorhabditis elegans) full-length proteins. Reactions with 26 mM MBP, 20 mM MBP-ScSET2, 30 mM MBP-CeSET-24, and 1 μg histones. Error bars represent mean ± SD. n = 4 independent replicates. The corresponding data points are shown. Statistical significance was assessed with two-way ANOVA multiple comparisons. ns, not significant; ****p value < 0.0001. MBP, Maltose-Binding protein. Source data and exact p values are provided as a Source Data file.
