Fig. 3: SET-24 is required for RNAi inheritance.
From: A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory
a Schematic representation of the experimental procedure. b Top: Representative images of GFP transgene activity in wild-type, hrde-1 and set-24 mutant germlines. Bottom: Percentage of derepressed individuals in every generation. Means and standard deviations are shown. Countings on >20 animals, experiments on N = 5 animal lineages per genotype, n = 3 independent replicates. c RT-qPCR analysis of GFP transgene (spliced) mRNA expression at every generation. Means and standard deviations are shown, n = 5 independent replicates for the ‘Before silencing’ and ‘G1–G7’, and n = 3 independent replicates for the ‘Empty vector’. d RT-qPCR measurement of the abundance of a representative anti-GFP 22G-RNA. Means and standard deviations are shown, n = 3 independent replicates. c, d Statistical significance was assessed with the two-sided unpaired t-test with Welch’s correction. Stars indicate p value, ns, not significant; *0.01 ≤ p value  <  0.05; **0.001 ≤ p value  <  0.01; *** p value  <  0.001. Comparisons were with wild-type strain. The corresponding data points are shown. Source data and exact p values are provided as a Source Data file.
