Fig. 4: SET-24 is a germline-specific factor and is localized to the nucleus.

a SET-24 localizes to germline nuclei. Representative immunofluorescence images of SET-24 localization (anti-GFP antibody, green) in dissected germlines of a SET-24::GFP strain. P-granules are stained with an anti-PGL-3 antibody, red, and DNA is stained with DAPI, blue. Numbered insets, zoom in on particular regions of the germline and on embryonic germ cells. n  =  3 independently imaged worms with similar results. b, c Representative images of C. elegans expressing SET-24::GFP and H2B::mCherry (b) or tagRFP::SYP-1 (c) in whole worms. n  =  10 independently imaged worms with similar results. d Pearson’s colocalization coefficiencies between SET-24::GFP and H2B::mCherry or tagRFP::SYP-1 within a single nucleus, measured using ImageJ. Means and standard deviations are shown. n = 20 nuclei from 10 different worms. Source data are provided as a Source Data file. e Schematic of deletions or mutations in set-24::gfp (left) and set-24::3xflag (right) strains. f Representative images of GFP expression and bright field in the indicated strains. The germline is outlined with white dashed lines. n  =  10 independently imaged worms with similar results. g Western blot analysis of FLAG expression in the indicated strains using anti-FLAG antibody (F1804). Red stars indicate the expected sizes of set-24(mj677)::3xflag and set-24(mj675)::3xflag. The same membrane was re-probed with anti-tubulin antibody (ab6160). n = 3 independent experiments with similar results. The uncropped and unprocessed versions of blots are provided in Source Data.