Fig. 5: Phosphoproteome, glycoproteome and proteome integrative analysis of mice aging cohort.

a Upset plot depicting significant phosphosites. b Median phosphosite fold change compared to the protein fold change in each compared group. c Heatmap of protein-level expression of MCP glycosidases across the different age groups. d Phosphorylation expression profiles of glycosidases Gys1 and Canx in each age group of MCP, comparison by ANOVA followed by Tukey’s two-sided multiple comparisons test, horizontal bars indicate median values (*P < 0.05, **P < 0.01). e Glycosylation/phosphorylation sites correlation in 24 M group. f Integrated KEGG enrichment analyses of proteome, phosphoproteome, and glycoproteome datasets. g The heatmap of changes in proteins, their glycosylation, and phosphorylation during aging in MCP, MCSF, and Mserum. Each group was analyzed using analysis of variance (ANOVA) followed by Tukey’s two-sided post-hoc multiple comparison test. Statistical significance between the groups (12 M/2 M, 24 M/12 M, and 24 M/2 M) is indicated by asterisks as follows: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.