Fig. 4: Functions enriched in two zyngematophytes challenged with osmotic stress.

Functional Enrichment of GO Terms and InterPro Domains During Osmotic Stress (FDR < 0.01). a Proteins were grouped into quadrants based on log2 fold changes (≥1 or ≤-1), highlighting significant changes in response to 0.15 M NaCl and 0.8 M mannitol treatments. Functional enrichment analysis was performed on each group to identify enriched GO terms and InterPro domains. The three terms enriched in both Mesotaenium and Zygnema are highlighted in orange. The P values were calculated by Fisher’s exact test. b Transcript temporal changes were clustered into patterns using a Gaussian Mixture Model, with normalized patterns further organized into modules. Functional enrichment analysis identified enriched terms for each module, with the top nine displayed. The colored shading shows the standard deviation of the unnormalized gene expression patterns that make up the modules. The center (symbol) in the error bands represents the mean.