Fig. 5: Integrated network of comparative proteomic and transcriptomic analysis in two zygnematophytes.

a This network illustrates the overlap of enriched Gene Ontology (GO) terms and protein domains across transcript modules and/or protein quadrants in Zygnema and Mesotaenium. Long names have been abbreviated for clarity. Module 7 of Zygnema and Module 9 of Mesotaenium show the highest overlap in number of shared functional terms, and both exhibiting transcriptional upregulation under osmotic stress. Terms only shared by these two modules are highlighted in green, with representative proteins common to both species written next to the term(s) and schematically depicted in the algae models (see b) (AHA = Plasma Membrane H + -ATPase, SuSy = Sucrose Synthase, TIP = Tonoplast Intrinsic Protein, SYT = Synaptotagmin). Terms and corresponding proteins showing opposing transcriptional regulation under osmotic stress are highlighted in red (upregulated in Mesotaenium) and blue (upregulated in Zygnema) within the schematic algae model (HSP20 = Small Heat Shock Protein 20, PetC = Component of the Cytochrome b6-f Complex). b Schematic Illustration of Mesotaenium and Zygnema cells with the proteins mentioned above shown. Proteins with significantly increased or decreased abundance (see Fig. 2) in both algae are depicted as well, in yellow and purple, respectively. Locations and functions depicted in the algae model are hypothetical.