Fig. 4: Fitness of fungal isolates in carbohydrate-rich and lipid-rich media in relation to their codon optimisation (S).

The maximum growth rate (A) and the lag time (B) are plotted against the S of the corresponding metabolic pathway (carbohydrate or lipid metabolism). The correlation for each pathway is colour-coded (blue, carbohydrates; orange, lipids), while the overall correlation of both datasets is indicated by the black dashed line. All correlations are derived from a linear regression model. Data are presented for isolates with consistent growth patterns across four independent replicates. Error bars represent the uncertainty in the estimated growth rate and lag time from the model fit for each replicate, as determined by omniplate. Isolates exhibiting abnormal growth or inconsistent replicates were excluded from the analysis.